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Plant leaves are studied by the analysis of the magnitude and phase spectra of their thickness mechanical resonances. These resonances appear at ultrasonic frequencies and have been excited and sensed using air-coupled ultrasounds. In spite of the complex leaf microstructure, the effective medium approach can be applied to solve the inverse problem, at least in the vicinity of the first thickness resonance. Results suggest that these resonances are sensitive to leaf microstructure, composition water content and water status in the leaf.