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This study applied the Frequency-domain Interferometric Imaging (FII) technique to Radio Acoustic Sounding System (RASS) measurement to improve the vertical resolution of temperature profiles obtained by RASS measurements. The FII technique was applied to RASS imaging measurements performed using the Middle and Upper atmosphere (MU) radar on October 29-31, 2006. The temperature profiles inside the radar range volume with the temporal resolution of 26 minutes were successfully estimated. The detailed temperature structure was revealed in the FII results. In particular, the detailed inversion layers structure inside the radar range gate were in good agreement with the simultaneous radiosonde results.