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Session Initiation Protocol (SIP) is adopted as the main signaling protocol for the IP Multimedia Subsystem (IMS). Large deployment of SIP servers in Next Generation Networks (NGN) requires studying the behavior and performance of SIP servers under both normal and overload conditions, which may happen in such networks with a vast number of users. In this paper a measurement-based study is performed to assess SIP proxy server performance under different configurations, and for different underlying transport protocols, for both inter-domain and intra-domain calls. Test results show the effectiveness of configuration and distance on SIP signaling performance. Therefore a great attention must be paid during SIP servers deployment.