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With the development of automatic identification technology, ultra high frequency radio frequency identification (UHF RFID) tags are used more and more widely in supply chain. One of the major challenges is the bulk reading. According to the most widely used UHF RFID standards, this paper proposed a multi-protocol RFID tag simulation platform which can simulate multiple UHF tag signals almost simultaneously. The simulation platform is based on a multi-processor hardware target which consists of a Texas Instruments TMS320C6713 digital signal processor and Altera Stratix II EP2S60 FPGA. The tag simulation platform could be used to verify UHF RFID system performance and collect processing data for further analysis to improve anti-collision or security algorithm. The platform also allows some configurations and shows very good performance.