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High throughput DNA methylation analysis on a droplet-in-oil polymerase chain reaction array

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8 Author(s)
Yi Zhang ; Dept. of Biomed. Eng., Johns Hopkins Univ., Baltimore, MD, USA ; Bailey, V. ; Puleo, C.M. ; Easwaran, H.
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We performed on-chip DNA methylation analysis using methylation-specific PCR (MSP) within a high throughput microfluidic droplet array. The device uses of the oil phase as a companion fluid for both sample actuation and compartmentalization. These technical advantages allow for infusion of minute amounts of sample for arrayed MSP analysis, without the added complexities inherent in microfluidic droplet-based studies. Ease of use of this micro device is exemplified by analysis of two tumor suppressor promoters, p15 and TMS1 using an on-chip methylation assay. These results were consistent with standard MSP protocols, yet the simplicity of the droplet-in-oil microfluidic PCR platform provides an easy and efficient tool for DNA methylation analysis in a large-scale arrayed manner.

Published in:

Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International

Date of Conference:

21-25 June 2009