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In this work, a novel device for batch separation of particles based on dielectrophoresis (DEP) is presented. The curved parts of serpentine micro-channel that causes performance deterioration in capillary electrophoresis (CE) due to the plug dispersion are effectively used in our design for dielectrophoretic separation. The top-and-bottom focusing electrodes are employed in the intermediate straight segments to bring all the particles to the same entering point for the next curve. The device was successfully fabricated by bonding two processed substrates with an SU-8 layer sandwiched in between. The experimental result we have achieved thus far is in good agreement with the numerical simulation result.