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This paper presents the design and implementation of a novel dielectrophoresis (DEP) system with spiral channels and concentric electrodes for high resolution cell separation applications. The device is fabricated with a 4 mask parylene process and the design is optimized in MATLAB Simulinkreg to confine the operation. Tests with micro particles of different sizes are performed to show size-based separation by dielectrophoresis. Proposed device is also tested with K562 leukemia cell lines to prove that they can be separated from healthy leukocytes due to the difference in their electrical properties.