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Discharge currents were measured on single pulse, multi-pulse glow dielectric barrier discharge (DBD) using high-frequency power supply in helium at atmospheric pressure. The influence of the applied voltage amplitude and frequency, as well as gap distance on multi-pulse glow DBD was discussed and analyzed. Conditions to form multi-pulse glow DBD were proposed. The results show that a higher amplitude of the applied voltage and a lower voltage frequency can be useful to form multi-pulse glow DBD. Moreover, the higher applied voltage is necessary to form multi-pulse glow DBD.