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A fluorescence correlation spectroscopy system based on two independent measurement volumes is presented. The optical setup and data acquisition hardware are detailed, as well as a complete protocol to control the location, size, and shape of the measurement volumes. A method that allows to monitor independently the excitation and collection efficiency distribution is proposed. Finally, a few examples of measurements that exploit the two spots in static and/or scanning schemes are reported.
Published in:
Review of Scientific Instruments
(Volume:80
,
Issue:
8
)
Date of Publication: Aug 2009
- Page(s):
- 083702 - 083702-8
- ISSN :
- 0034-6748
- Digital Object Identifier :
- 10.1063/1.3205447
- Product Type:
- Journals & Magazines
- Date of Current Version :
- 25 August 2009
- Issue Date :
- Aug 2009
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