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Special purpose 3-D reconstruction and restoration algorithms for electron microscopy of nanoscale objects and an enabling software toolkit

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4 Author(s)
Cory J. Prust ; Electrical and Computer Engineering, Purdue University, 465 Northwestern Avenue, West Lafayette, IN 47907, USA ; Kang Wang ; Yili Zheng ; Peter C. Doerschuk

Two algorithms are described for electron microscopy tasks: (1) 3-D reconstruction of an infectious bacteriophage, including the tail, based on a set of single-particle cryo electron microscopy images of identical phage particles with one image per particle and (2) restoration, resolution enhancement, and classification of spherical viruses in a cell based on a tomographic reconstruction of the entire cell. While different tasks, the statistical algorithms, which are maximum likelihood estimators, have extensive commonality. In order to provide solutions for such tasks in a timely fashion, a software toolkit has been created, based on the commonality, for writing parallel software systems to solve these problems.

Published in:

2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro

Date of Conference:

June 28 2009-July 1 2009