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A hardware and software solution is proposed for the development of a DSP-operated cell/particle counting and sizing system. LabVIEW, which is a graphical programming language, was adopted as the DSP programming platform. Special attention was paid to data reduction, noise elimination, baseline restoration, and pulsewidth discrimination. Global accuracy is verified based on externally generated test signals and using particle size standards. The hardware and software, with minor modifications, are also suitable as a detection system for cell/particle chromatography and flow cytometry.