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This paper reports a novel method for Nucleus and Micro Nucleuses segmentation. These biological structures are very handy to biologists for relieving structural chromosome aberration. The adopted method consists into a pipeline of advanced computer vision algorithms some of them specifically tailored for the current segmentation problem. Starting from weak hypotheses on size, shape and colour of Micro Nucleuses it is possible to efficiently segment all the image features by computer vision approach. Experimental results on real images are reported.