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The Enhancement of Drug Sensitivity of Human AFP-Producing Gastric Carcinoma Cell Line FU97 to Arsenic Trioxide Using AFP as Targeting siRNA

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5 Author(s)
Yan-Fei Jia ; Central Lab., Shandong Univ., Jinan, China ; Yan Zheng ; Xiao-Li Ma ; Zhan-Tao Su
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AFP-producing gastric carcinoma(APGC) are known to have a poorer prognosis and to show a higher incidence of liver metastasis than ordinary gastric carcinoma. Therefore, surgical resection, chemotherapy or radiotherapy is usually not satisfactory. In recent years, effects of arsenic trioxide (As2O3) on cell proliferation and induced apoptosis of solid tumors have been widely studied, while little is known about the APGC. This study was undertaken to delineate the effect of As2O3 on AFP gene expression ,the cell growth and apopotosis ,and to study the effect of the siRNA targeting AFP gene on the drug sensitivity of APGC cell line FU97 to As2O3. AFP gene sequence-specific siRNA expression vector (AFP-siRNA) and sequence-unrelated control were transfected into FU97. At 6h after transfection ,the cells were cultured with 2 mumol/L As2O3, with corresponding untreated cells serving as controls.The growth inhibition rate on FU97 was determined by MTT assay; Apoptosis of FU97 was investigated by DNA gel electrophore; The expressions of AFP,VEGF were determined by realtime-PCR, western blot, ELISA and chemistry luminescence. The combination of AFP-siRNA with arsenic trioxide resulted in significantly inhibitory action on growth of FU97 cells ,as well as descending of expression of AFP and VEGF.The effective siRNA targeting AFP can increase the drug sensitivity of the FU97 to arsenic trioxide.

Published in:

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on

Date of Conference:

11-13 June 2009