By Topic

Preliminary Study on the Expression of BGT Gene in Transgenic Birch

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)
Fan-suo Zeng ; Coll. of Life Sci., Northeast Forestry Univ., Harbin, China ; Hong-Cui Zhao ; Ying Xin ; Ya-guang Zhan
more authors

In this paper, Southern blot analysis of the transgenic birch (Betula platyphylla Suk.) plants indicated that the the copy number of bgt varies from one to four. Of which 18.2 percent were single copy. Transcriptional expression of bgt gene in transgenic birch plants was analyzed by Northern blot. TGS has been found in four transgenic lines with one, three and four copies of bgt. Bgt gene was inserted into the prokaryotic vector pET 28a and transferred into E.coli BL21 (DE3) for expression of BGT fusion protein. After overnight cleavage with Thrombin and purification by immobilized metal ion affinity chromatography, the final BGT proteins were obtained with purity of 90%. The ELISA result showed that the BGT protein expression level in the transgenic birch plants ranged from 0.000% to 0.283% of total soluble protein. No significant correlation was found between copy number and expression level of bgt gene.

Published in:

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on

Date of Conference:

11-13 June 2009