Cart (Loading....) | Create Account
Close category search window
 

On Optimization Study of Cultivation Conditions of a Flocculant-Producing Strain and 16s rDNA-Sequential Analysis

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)
Li He ; Biotechnol. Core Facilities, Central South Univ. of Forestry & Technol., Changsha, China ; Li Lin ; Zhou Guoying ; Hao Yan
more authors

The article introduces the authors'research of an optimum method on the cultivation conditions of a flocculant- producing Strain. Using single factor experiments and orthogonal test, the influence of temperature, PH value and rotational speed on synthesis of microbial flocculants, and optimized culture conditions were studied; 16S gene of the strain was amplified and sequenced.The sequencing was BLAST search in Genebank and phylogenetic tree was constructied to make certain the taxonomic status of the strain. The results: the optimum culture conditions of the flocculant-producing strain to produce microbial flocculants were 28degC, initial pH 8, 160r/min; The effects of other factors needs further study. The 16S gene sequence of the strain had the high homology to Bacillus megaterium(>99%), the phylogenetic tree also showed that the strain and Bacillus megaterium were in the same branch. We could draw a conclusion that the microbial flocculants producing strain was identified as Bacillus megaterium.

Published in:

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on

Date of Conference:

11-13 June 2009

Need Help?


IEEE Advancing Technology for Humanity About IEEE Xplore | Contact | Help | Terms of Use | Nondiscrimination Policy | Site Map | Privacy & Opting Out of Cookies

A not-for-profit organization, IEEE is the world's largest professional association for the advancement of technology.
© Copyright 2014 IEEE - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.