By Topic

Effect of Exogenous Cholesterol on Cholesterol Accumulation and mRNA Expression of SREBP-2 and HMGR in Goose Primary Hepatocytes

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)
Han Chun-Chun ; Key Lab. of Animal Genetic Resources, Sichuan Agric. Univ., Ya''an, China ; Wang Ji-Wen ; Li Liang ; Zhang Zhong-Xian
more authors

In order to understand the effect of exogenous cholesterol on cholesterol accumulation and mRNA expression of SREBP-2 and HMGR in goose, primary hepatocytes from Landes Goose (Anser anser) and Tianfu Meat Goose (Anser cygnoides) were isolated and treated with the exogenous cholesterol. Cholesterol accumulation, and gene expression levels of SREBP-2 and HMGR were measured. The results indicated exogenous cholesterol cultured with hepatocytes for 72 h had no evident effect on TC content in hepatocytes. We found TC content increased before 24 h, decreased after 48 h. Exogenous cholesterol could decrease the mRNA level of SREBP-2 and HMGR. The correlation analysis indicated the mRNA expression of SREBP-2 was positively correlated to that of HMGR. In conclusion, exogenous cholesterol could regulate the cholesterol content by decreasing the gene expression of SREBP-2 and HMGR in goose primary hepatocytes.

Published in:

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on

Date of Conference:

11-13 June 2009