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Cloning and Sequence Characterization of SFBB (S-Locus F-Box Brothers)-Gamma Genes in Chinese Sand Pear (Pyrus pyrifolia Nakai.)

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5 Author(s)
Tan Xiaofeng ; Key Lab. of Non-wood Forest Product of Forestry Minist., Central South Univ. of Forestry & Technol., Changsha, China ; Zhang Lin ; Long Hongxu ; Hu Jiao
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Gametophytic self-incompatibility (GSI) is a genetically controlled mechanism to prevent inbreeding and promote out-crossing. It is controlled by a single multi-allelic S- locus which contains two separate genes: one stylar S-RNase gene and one pollen S-gene. In gametophytic self-incompatible Japanese pear (Pyrus pyrifolia Nakai.), SFBB-gamma genes have been identified as good candidates for pollen S-genes. To investigate sequence characters of the possible pollen S-gene in Chinese sand pear, degenerate primers PSFBG-F and PSFBG-R were designed for cloning the SFBB-gamma homologies in eight Chinese sand pear cultivars. By genomic PCR, sequencing and sequence analysis, a total of nine distinct sequences were identified which displayed the basic structural characterization of SFBISLF genes, i.e. an F-box motif and four variable regions. Of the nine sequences, five equaled to reported Japanese pear SFBB-gamma genes (S1, S2, S4, S5 and S7) and the other four were considered to be new ones which were named SFBB12-gamma, SFBB13-gamma, SFBBlS-gamma and SFBB22-gamma. The four new SFBB-gamma genes were expressed specifically in pollen grains. At the deduced amino acid level, they shared 20.4% to 97.7% similarities with other SFB/SLFs of rosaceous plants. The four SFBB-gamma genes cloned in this work will be useful for studying the interaction between SFBs and S-RNases, and clarifying the mechanism of GSI at the molecular level in Chinese pear.

Published in:

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on

Date of Conference:

11-13 June 2009