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Evaluation of ship handling training has usually depended on professionals who have a lot of experience on board. We are attempting to evaluate a ship navigator's mental workload (stress) based on a physiological index. The physiological indices, heart rate variability and nasal temperature, are good indices of the stress found in ship handling; however, it is best if we get response and evaluation results quickly on the spot. A recent study shows salivary amylase activity is reflected by the sympathetic nervous system. This paper proposes that salivary amylase activity shows a ship navigator's stress during ship handling.