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The real-time data communication of real-time operation system is a difficult point. This paper analyzes the functions and characteristics of embedded real-time operation system VxWorks and CAN field bus, and makes use of CAN field bus to realize the data communication between embedded real-time operation system VxWorks and work-site MCU (micro-controller unit). The applications of CAN bus can satisfy the reliability and real-time request of data communication completely. The general steps of CAN bus applications offer reference for CAN bus applications in real-time operation system.