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An easy cell-based microchip assay method for a CYP1A1-mediated drug metabolism using adhesive cells, HepG2

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5 Author(s)
Kato, Masaru ; Center for NanoBio Integration, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan ; Yamamoto, Tatsuhiro ; Sekimoto, Masashi ; Degawa, Masakuni
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We have developed a convenient cell-based assay method using a microchip. In the method, adhesive cells, HepG2, were cultured in the conventional culture dish containing glass disks and then the disks covered with the HepG2 were transferred to the microchip for cell assay. Activity of ethoxyresorufin O-deethylation (EROD), which is mainly mediated by cytochrome P4501A1 (CYP1A1), in HepG2 was measured. Treatment of HepG2 with 3-methylcholanthrene, a CYP1A1 inducer, resulted in significant increase in EROD activity.

Published in:

Journal of Applied Physics  (Volume:105 ,  Issue: 10 )

Date of Publication:

May 2009

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