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In this study, 27 young and healthy subjects was listening to the classical and Turkish music samples which are believed to changeable the subject's emotional state. Their effects on autonomic nervous system (ANS) are investigated by using the heart rate variability (HRV) analysis which is accepted as a non-invasive indicator of ANS. The electrocardiogram (ECG) and respiration records were made before and during the listening music samples. Heart rate variability (HRV) signals are obtained form records and power spectral densities (PSD) of HRV are estimated. According to the results of calculated parameters, during the listening to classical music samples some statistically significant changes were occurred in sympathetic and parasympathetic activities of ANS. Turkish music samples were not causes significant differences on the ANS related HRV parameters.