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A Simple, Low-Cost Double Beam Spectrophotometer for Colorimetric Detection of Nitrite in Seawater

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4 Author(s)
Weidong Gong ; Nanoscale Syst. Integration Group, Univ. of Southampton, Southampton, UK ; Matt Mowlem ; Michael Kraft ; Hywel Morgan

This paper reports on the optical detection system of a prototype nitrite sensor for seawater analysis. The sensor uses a colorimetric Griess reaction that produces an Azo dye with an absorption peak at 540 nm. A simple low-cost double beam spectrophotometer using a green light emitting diode (LED) light source, two integrated photo detectors and lock-in detection is described. A log-ratio amplifier is used to produce an output proportional to absorbance. The optical devices were mounted in a cast resin block to prevent shock and vibration. Experimental results show the detection system has a limit of detection of 0.1 muM.

Published in:

IEEE Sensors Journal  (Volume:9 ,  Issue: 7 )