By Topic

Experimental Study of Human Bone Marrow Mesenchymal Stem Cells Transfected with Vascular Endothelial Growth Factor Gene in Vitro

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

4 Author(s)
Dewu Liu ; Nanchang Univ. Nanchang, Nanchang ; Xiangrong Zhang ; Guanghua Guo ; Yan Peng

Vascular endothelial growth factor 165 (VEGF165) is one of the most prominent vascular growth factors that has the potential to accelerate wound healing by promoting the regeneration of capillary vasculature. However, the short biological half-live of growth factors may impose severe restraints on their clinical applications. Here, we investigate the feasibility and safety of human bone marrow derived mesenchymal stem cells (MSCs) transfected with VEGF165 gene so as to provide a new method for clinical skin repair in the future. The MSCs were harvested, isolated and purified by combining density gradient centrifugation with adhering method. The pShuttle-CMV/VEGF165 was introduced into cultured MSCs through liposome-mediated transfection. The VEGF165 gene and protein expression in transfected MSCs and untransfected MSCs were determined by reverse transcription-polymerase chain reaction (RT-PCR), Enzyme-linked Immunoadsorbent Assay (ELISA) and western blot analysis respectively. We found that the expression of VEGF165 mRNA and protein in transfected MSCs were much higher than those in the untransfected MSCs. These results suggest that human bone marrow derived mesenchymal stem cells can be successfully transfected with vascular endothelial growth factor 165 gene mediated by liposome in vitro. The present study has laid a foundation for potential use of VEGF165 gene transfection to accelerate the vascularization of tissue engineered skins grafting.

Published in:

Future BioMedical Information Engineering, 2008. FBIE '08. International Seminar on

Date of Conference:

18-18 Dec. 2008