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In order to improve the system performance over wireless fading channel, transmit diversity technique has been proven as an attractive and effective solution especially for broadcasting systems. In this paper, a PN sequence design scheme based on both cyclic-shift m-sequences and space-time block coded (STBC) m-sequences for time domain synchronous orthogonal frequency-division multiplexing (TDS-OFDM) system with transmit diversity is proposed. Based on this novel design, flexible channel estimation which provides a trade-off between maximum delay spread and maximum Doppler spread over double-selective channel is investigated and a radix-2 fast Fourier transform (FFT) approach is adopted for implementation from the viewpoint of computational complexity. Simulations show that the performance of the proposed transmit diversity scheme is better than conventional one and perform well under different propagation conditions.