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A novel microfluidic platform is presented for sorting by size dissociated neurons, glia and stem cells from biopsies of the central nerve system. A highly biocompatible aqueous polymer solution was used in hydrodynamic spreading controlled cell separation. Before cell separation, particles were used for demonstration. To verify the results the fractions were studied using flow cytometry. Further, they were cultured and differentiated. The study indicated that the technique is ready for biological study and that it has a high potential for applications in neural cell regeneration therapy.