Cart (Loading....) | Create Account
Close category search window

Measurement of rapid in vitro response to an agonist of ion channel forming cell surface receptors

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $31
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

1 Author(s)
Marque, Jeffrey ; School of Applied and Engineering Physics, Cornell University, Ithaca, NY

Your organization might have access to this article on the publisher's site. To check, click on this link: 

We describe an instrument that is used for spatially homogeneous, submillisecond application of low‐molecular‐weight substrates (hundreds of daltons) in the neighborhood of a single biological cell and the measurement of the subsequent response of the cell to the substrate. Substrate application is accomplished by ultraviolet photolysis of caged substrate. Cell response is measured, in our particular case, as current flux into the cell in response to membrane conductance changes caused by substrate binding to cell surface receptors. We present data that show fast channel‐opening kinetics of nicotinic acetylcholine receptors on the surface of a BC3H1 cell, a transformed cell line from mouse brain, and we discuss the measurements of ultraviolet‐induced cell damage and radiation fluence at the cell surface. The technique described is also useful for nonbiological studies requiring known fluences applied to microscopic samples.

Published in:

Review of Scientific Instruments  (Volume:60 ,  Issue: 11 )

Date of Publication:

Nov 1989

Need Help?

IEEE Advancing Technology for Humanity About IEEE Xplore | Contact | Help | Terms of Use | Nondiscrimination Policy | Site Map | Privacy & Opting Out of Cookies

A not-for-profit organization, IEEE is the world's largest professional association for the advancement of technology.
© Copyright 2014 IEEE - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.