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Light-Addressed Stimulation Under \hbox {Ca}^{\bf 2+} Imaging of Cultured Neurons

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5 Author(s)
Suzurikawa, J. ; Grad. Sch. of Inf. Sci. & Technol., Univ. of Tokyo, Tokyo, Japan ; Nakao, M. ; Jimbo, Yasuhiko ; Kanzaki, R.
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Light addressing is an emerging and sophisticated technique that can induce pinpoint and/or patterned neuronal activation in cultured neurons. We previously developed a light-addressable electrode using hydrogenated amorphous silicon (a-Si:H), which was sandwiched between a tin oxide (SnO2) substrate and a passivation layer of zinc antimonate (ZnOSb2O5) dispersed epoxy. This research developed an experimental system that simultaneously implemented light-addressed stimulation and Ca2+ imaging of neuronal activities. The translucent and thin laminated structure of our electrode permitted optical accesses from two directions: Ca2+ imaging from above and light addressing from beneath. The submillisecond bright/dark switching property of our electrode offered light-addressed stimulation without causing interference with Ca2+ imaging. To provide patterned illumination for light addressing, a digital micromirror device was installed in the system as an active photomask. The system could induce pinpoint neuronal activation at a cellular level.

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Biomedical Engineering, IEEE Transactions on  (Volume:56 ,  Issue: 11 )