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Fourier imaging study of efficient near-field optical coupling in solid immersion fluorescence microscopy

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4 Author(s)
Yoshita, Masahiro ; Institute for Solid State Physics, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581, Japan ; Koyama, Kazuko ; Baba, Motoyoshi ; Akiyama, Hidefumi

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We measured images and Fourier images of fluorescence for 0.11- and 0.22-μm-diameter dye-doped polystyrene microsphere beads on a solid immersion lens, and experimentally verified strongly angle-dependent fluorescence intensities due to efficient near-field optical coupling in solid immersion fluorescence microscopy. The results are interpreted in comparison with calculated emission patterns of an emission dipole placed near a solid surface, which establish a basic model for high-collection efficiency in solid-immersion fluorescence microscopy. © 2002 American Institute of Physics.

Published in:

Journal of Applied Physics  (Volume:92 ,  Issue: 2 )

Date of Publication:

Jul 2002

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