We have studied the crystallographic domain structure dependent anisotropy in the magnetotransport properties of epitaxial thin films of the conductive ferromagnetic oxide SrRuO3. Single domain SrRuO3 thin films on miscut (001) SrTiO3 substrates exhibit a strong anisotropy in magnetization and magnetoresistance that reflects the inherent magnetocrystalline anisotropy of the material. In contrast, the SrRuO3 thin film on (001) LaAlO3 substrate shows identical magnetization and magnetotransport properties in two orthogonal directions on the film due to the presence of 90° domains in the plane. For both films, large negative magnetoresistance effects (∼10%) are observed when the applied magnetic field is parallel to the current due to the suppression of spin fluctuations near Tc and the anisotropic magnetoresistance effect at low temperatures. © 1998 American Institute of Physics.