Cart (Loading....) | Create Account
Close category search window

Capillary-based, high-pressure chamber for fluorescence microscopy imaging

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $31
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

4 Author(s)
Raber, Erica C. ; Department of Physics, Miami University, Oxford, Ohio 45056 ; Dudley, Jonathan A. ; Salerno, Michael ; Urayama, Paul

Your organization might have access to this article on the publisher's site. To check, click on this link: 

We present a high-pressure chamber suitable for the fluorescence microscopy imaging of cellular systems, consisting of a quartz capillary attached to high-pressure tubing with the capillary walls acting as the optical window and mechanical support. The chamber withstands pressures up to 1000 atm, spanning most of the biosphere’s pressure range. We validate the chamber for high-pressure studies by using a pH-sensitive fluorophore to sense the pressure-induced acidification of a phosphate buffer. Next, because the capillary walls result in image aberrations, we demonstrate the ability to use spatial deconvolution to improve image resolution. Capillary wall thicknesses are less than 0.5 mm, making the chamber compatible with high-numerical-aperture optics.

Published in:

Review of Scientific Instruments  (Volume:77 ,  Issue: 9 )

Date of Publication:

Sep 2006

Need Help?

IEEE Advancing Technology for Humanity About IEEE Xplore | Contact | Help | Terms of Use | Nondiscrimination Policy | Site Map | Privacy & Opting Out of Cookies

A not-for-profit organization, IEEE is the world's largest professional association for the advancement of technology.
© Copyright 2014 IEEE - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.