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Construction of multiphoton laser scanning microscope based on dual-axis acousto-optic deflector

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5 Author(s)
Lv, Xiaohua ; The Key Laboratory of Biomedical Photonics of Ministry of Education, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, People’s Republic of China ; Zhan, Chen ; Zeng, Shaoqun ; Chen, Wei R.
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The construction of a multiphoton laser scanning microscope based on a dual-axis acousto-optic deflector (AOD) is described. Without mechanical inertia and physical movement of the scanner, this system has a high random-addressing and data-acquisition rate of 10 μs pixel. By proper compensation of the dispersion of the AODs, the spatial resolution of the system is 0.5 μm for lateral direction and 1.5 μm for axial direction. Fluorescence images are acquired to demonstrate these imaging performances.

Published in:

Review of Scientific Instruments  (Volume:77 ,  Issue: 4 )

Date of Publication:

Apr 2006

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