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An integral system was developed for the measurement and analysis of wall shear stress data from a two-phase boundary layer flow. Three different and independent measurement techniques are incorporated in a synchronized acquisition system. The three measurement techniques are the particle tracking velocimetry, a differential pressure transducer, and an optical wall shear stress sensor. Each of these techniques provides complementary information that helps in the description and understanding of the phenomena involved in a two-phase boundary layer. The practical implementation of the integral measurement and analysis system is demonstrated with the measurement of the wall shear stress of a microbubble laden boundary layer flow in a channel. The agreement in the results of the wall shear stress from the three synchronized measurements techniques was 93% for the single-phase case and 92.8% for the two-phase flow measurements. Moreover, independent measurements with each technique show no appreciable change in the values obtained when they were all synchronized. The advantages and limitations of each individual technique are discussed for measurement and analysis of two-phase flow data, as well the advantages of having an integral system that combines the information from all measurement techniques at the same time.