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Application of double-cell arrangement in planar laser-induced fluorescence experiments

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3 Author(s)
Golnabi, H. ; Institute of Water and Energy, Sharif University of Technology, P. O. Box 11365-8639, Tehran, Iran ; Ahmadi, H. ; Razani, M.

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This paper describes a double-cell method, which offers some advantages in image capturing and analysis of the planar laser-induced fluorescence (PLIF) experiments. Double static-cell and circulating-static designs are tested and the fluorescence results are reported. The static-static design offers some advantages when comparing the fluorescence signals for two different dyes, the effect of additives, and of oxidation, whereas the circulating-static design provides an effective method to study the thermal contributions of the measured signal, the photostability of a particular dye, the effect of adding agents, and the quenching effects on a dye under study. In this experiment Rhodamine-WT (Rh-WT) and Resorufin (RSRF) dye solutions are tested and PLIF results are reported. The photostability of both dyes is investigated and the oxidation process of fluorescent probe molecule RSRF is also demonstrated. Results obtained for these dyes are very interesting and verify that such a method can be used effectively for investigating different interesting phenomenon affecting the fluorescence emission/extinction. It is also possible to compare the fluorescence yields for different dyes in different solvents and at different molar concentrations.

Published in:

Review of Scientific Instruments  (Volume:76 ,  Issue: 8 )

Date of Publication:

Aug 2005

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