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Automated analysis of nerve-cell images using active contour models

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3 Author(s)
Ying-Lun Fok ; Dept. of Comput. Sci., Hong Kong Univ. of Sci. & Technol., Kowloon, Hong Kong ; Chan, J.C.K. ; Chin, R.T.

The number of nerve fibers (axons) in a nerve, the axon size, and shape can all be important neuroanatomical features in understanding different aspects of nerves in the brain. However, the number of axons in a nerve is typically in the order of tens of thousands and a study of a particular aspect of the nerve often involves many nerves. Potentially meaningful studies are often prohibited by the huge number involved when manual measurements have to be employed. A method that automates the analysis of axons from electron-micrographic images is presented. It begins with a rough identification of all the axon centers by use of an elliptical Hough transform procedure. Boundaries of each axons are then extracted based on active contour model, or snakes, approach where physical properties of the axons and the given image data are used in an optimization scheme to guide the snakes to converge to axon boundaries for accurate sheath measurement. However, false axon detection is still common due to poor image quality and the presence of other irrelevant cell features, thus a conflict resolution scheme is developed to eliminate false axons to further improve the performance of detection. The developed method has been tested on a number of nerve images and its results are presented

Published in:

Medical Imaging, IEEE Transactions on  (Volume:15 ,  Issue: 3 )

Date of Publication:

Jun 1996

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