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Fluorescence lifetime imaging using a compact, low-cost, diode-based all-solid-state regenerative amplifier

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6 Author(s)
Mendez, E. ; Blackett Laboratory, Imperial College London, Prince Consort Road, London, SW7 2BW, United Kingdom ; Elson, D.S. ; Koeberg, M. ; Dunsby, C.
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A fluorescence lifetime imaging (FLIM) system is described that utilizes a new compact and low-cost ultrafast laser source based on a gain-switched laser diode-seeded all-solid-state Cr:LiSAF regenerative amplifier that has been designed for this application. The pulse parameters of this source (0.5 μJ, 827 nm, 100 ps, 5 kHz) are shown to be appropriate to time-domain FLIM using a gated optical intensifier and the application to functional imaging of biological tissue is demonstrated, as well as the first evaluation of organic light emitting diodes using FLIM. © 2004 American Institute of Physics.

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Review of Scientific Instruments  (Volume:75 ,  Issue: 5 )