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Flow cell for in situ optical microscopy in water at high temperatures and pressures up to supercritical state

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2 Author(s)
Deguchi, Shigeru ; The DEEPSTAR Group, Japan Marine Science and Technology Center (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan ; Tsujii, Kaoru

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A high temperature and pressure flow cell for optical microscopy has been developed. The cell is designed to allow observations at temperatures and pressures above the critical point of water (374 °C, 22.1 MPa) up to 400 °C and 35 MPa. Due to the small dimension and the effective cooling system, the cell can be installed on a conventional inverted optical microscope, and observations can be done with the optical resolution of 2 μm. A microscope system incorporating the present cell has been used successfully to observe micro-organisms under hydrothermal conditions including supercritical water.© 2002 American Institute of Physics.

Published in:
Review of Scientific Instruments  (Volume:73 ,  Issue: 11 )

Date of Publication: Nov 2002

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