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Development of an isothermal titration microcalorimetric system with digital control and dynamic power peltier compensation. II. Characterization and operation mode. Myoglobin adsorption onto polymeric latex particles

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3 Author(s)
Velazquez-Campoy, A. ; Departamento de Fı´sica Aplicada, Facultad de Ciencias, Universidad de Granada, E-18071 Granada, Spain ; Lopez-Mayorga, O. ; Cabrerizo-Vı lchez, M.A.

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In a previous article a comprehensive description of an isothermal titration microcalorimeter with Peltier compensation was reported. This work deals with the characterization procedure and the operation mode. The transfer function parameters (time constants, calibration constants, and thermal properties of the system components) have been determined using a rigorous physical model for the microcalorimeter. To check the good performance of the instrument, titration experiments of cytidine and adenosine protonation have been carried out. Finally, as an example of the instrument applicability, differential heat measurements of myoglobin adsorption onto polymeric (polystyrene) latex particles are presented. © 2000 American Institute of Physics.

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Review of Scientific Instruments  (Volume:71 ,  Issue: 4 )