Cart (Loading....) | Create Account
Close category search window
 

Design and construction of a versatile scanning near‐field optical microscope for fluorescence imaging of single molecules

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $31
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

4 Author(s)
Tarrach, G. ; Institut für Physik, Universität Basel, Klingelbergstrasse 82, CH‐4056 Basel, Switzerland ; Bopp, M.A. ; Zeisel, D. ; Meixner, A.J.

Your organization might have access to this article on the publisher's site. To check, click on this link:http://dx.doi.org/+10.1063/1.1145471 

A scanning near‐field optical microscope (SNOM) for fluorescence imaging of single molecules requires efficient optical signal detection and background rejection combined with long‐term stability and high spatial resolution. These requirements are dictated by the extremely low fluorescence signal of an individual dye molecule. We have built a SNOM that meets these requirements by combining a rigid and versatile near‐field optical scanner with confocal detection optics. The relevant design parameters are discussed in detail. The near‐field part of the microscope is based on a commercially available fiber aligner for coarse approaching the sample with respect to the tip. It also permits us to select a specific sample area in a range of 3 mm×3 mm with a nominal resolution of 10 nm. The tip–sample separation is probed by shear‐force detection using a fiber‐optical interferometer, which gives an excellent signal‐to‐noise ratio. The high versatility of this SNOM is demonstrated with measurements of a transparent dielectric grating and by imaging the fluorescence from individual rhodamine‐6G molecules with a spatial resolution of ≊160 nm. © 1995 American Institute of Physics.

Published in:

Review of Scientific Instruments  (Volume:66 ,  Issue: 6 )

Date of Publication:

Jun 1995

Need Help?


IEEE Advancing Technology for Humanity About IEEE Xplore | Contact | Help | Terms of Use | Nondiscrimination Policy | Site Map | Privacy & Opting Out of Cookies

A not-for-profit organization, IEEE is the world's largest professional association for the advancement of technology.
© Copyright 2014 IEEE - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.