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Novel optical device for simultaneous measurements of Raman and absorption spectra: Application to photolabile reaction intermediates of hemeproteins

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2 Author(s)
Ogura, Takashi ; Institute for Molecular Science, Okazaki National Research Institutes, Myodaiji, Okazaki 444, Japan ; Kitagawa, Teizo

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Resonance Raman studies on transient molecules have raised a problem about characterization of a photolabile species. Therefore, in order to observe the Raman and absorption spectra of the same spatial region of the sample at the same time, we have constructed a novel device and applied it successfully to monitor the transient species involved in an enzymic reaction of horseradish peroxidase and in photodissociation of the carbon monoxide adduct of hemoglobin.

Published in:

Review of Scientific Instruments  (Volume:59 ,  Issue: 8 )

Date of Publication:

Aug 1988

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