We are currently experiencing intermittent issues impacting performance. We apologize for the inconvenience.
By Topic

Nanoshell build-up onto non-fixed human red blood cells through the layer-by-layer self-assembly technique for their immunocamouflage

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

4 Author(s)
Mansouri, S. ; Dept. of Biomed. Eng., McGill Univ., Montreal, QC ; Merhi, Y. ; Winnik, F.M. ; Tabrizian, M.

In this study, non-fixed red blood cells (RBCs) were coated with a polymeric shell via the layer-by-layer (LbL) self-assembly technique for their potential application in medical blood transfusion. The polymeric shell is achieved through the alternate adsorption of two oppositely charged polymers; chitosan-graft-phosphorylcholine (CH-PC) and alginate (ALG). The process of film formation was followed by measuring the zeta potential revealing a charge alternation upon the addition of the polyelectrolytes. The integrity of the coated RBCs was assessed with optical microscopy demonstrating similar shapes to uncoated RBCs. Moreover, the percentage of released hemoglobin and adenosine-5'-triphosphate (ATP) in the supernatant were measured and compared to the non-coated RBCs. The results showed that the layers built on the RBCs demonstrated no changes in cellular morphology and integrity. Most importantly, an agglutination test was performed to assess antibodies interaction with coated RBCs. They showed no aggregation suggesting a decrease in antibody recognition of coated RBCs. In summary, a system of two different polymers is successfully able to protect the cells from hemolysis and prevent recognition by the antibodies. This would offer a great opportunity for producing universal RBCs.

Published in:

Bioengineering Conference, 2009 IEEE 35th Annual Northeast

Date of Conference:

3-5 April 2009