Cart (Loading....) | Create Account
Close category search window

Treating mesenchymal stem cells with hepatocyte growth factor enhances cardiac markers

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

2 Author(s)
Guyette, J.P. ; Dept. of Biomed. Eng., Worcester Polytech. Inst., Worcester, MA ; Gaudette, G.R.

Human mesenchymal stem cells (hMSCs) derived from adult bone marrow are multipotent cells shown to have increased differentiation potential when cultured in reduced-oxygen (2% O2) environments. Hepatocyte growth factor (HGF) is a mesodermal cytokine critical for organ formation during embryogenesis, including cardiogenesis. In this work, we tested the hypothesis that culturing hMSCs in reduced O2 conditions with media supplemented with HGF increases the expression of cardiac myocyte markers. RT-PCR demonstrated expression of myocyte specific alpha-Actinin (ACTN2) and myocyte enhancement factor 2C (MEF2C) in reduced-O2/HGF+ treated cells. Real time PCR showed an 11-fold increase in ACTN2 and 2-fold increase in MEF2C in reduced-O2/HGF+ treated cells compared to hMSCs cultured in atmospheric-O2/HGF-conditions. These results suggest that the combination of low oxygen and HGF may increase the cardiac differentiation capabilities of hMSCs.

Published in:

Bioengineering Conference, 2009 IEEE 35th Annual Northeast

Date of Conference:

3-5 April 2009

Need Help?

IEEE Advancing Technology for Humanity About IEEE Xplore | Contact | Help | Terms of Use | Nondiscrimination Policy | Site Map | Privacy & Opting Out of Cookies

A not-for-profit organization, IEEE is the world's largest professional association for the advancement of technology.
© Copyright 2014 IEEE - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.