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The experiment of practical course teaching of information-based enterprise management has been summarized in this paper. The aims of the practical course are to cultivate the students to build the conceptual framework and systematical understanding of the information-based enterprise management, to promote the students' comprehension on the behavior of enterprise management from their individual life experience and to train the students learning to solve problems in the enterprise management by using tools of computer and application software. The major approach is to arouse the imagination and creativity of the students to set their course study objects. With the object of solving problems in enterprise management, the students adequately use the Web resource, search the informationization tools and learn to apply them to information-based enterprise management. Through the practical course, the integrated ability of using tools to solve problems has been increased. The methodology of PDCA has been used in the process of course teaching and nicer effect has been acquired.