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In previous works, the performance of DS-UWB (Direct Sequence- Ultra Wide Band) systems in a multiuser scenario with binary pulse amplitude modulation has been studied. In most of these works, independent identically distributed (i.i.d) random sequences have been considered and no optimization of the DS-UWB performance with respect to the choice of spreading sequences has been done. In this paper, we investigate an analysis of multi-user interference (MUI) term in three different families of traditional spreading sequences; i.i.d, Gold and Kasami sequences on Rayleigh and AWGN (additive white gaussian noise) channel. Then, we evaluate the bit error rate (BER) performance of conventional receiver on both channels to confirm the results found about MUI.