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Instrumental development attachable to high magnification microscopes for obtaining totally focalized images

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3 Author(s)
Navas, Francisco Javier ; Department of Physical Chemistry, Science Faculty, University of Cadiz, Pol. Río San Pedro s/n, Puerto Real, Cádiz 11510, Spain ; Kulawik, Moritz ; Martin, Joaquin

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A complete system (instrumentation and software) for acquiring micrograph images with an extended depth of focus and with no alteration to the original chromaticity has been developed. The buildup system includes (a) the electromechanical components to be coupled to a commercial microscope in order to improve it, (b) a computer program with which the focusing distance can be micrometrically modified at the same time that a set of digital images can be acquired, and (c) software that permits the in-focus pixels of each image to be extracted and incorporated into a global image with an extended depth of focus greater than that supplied by the optical characteristics of the objective used to visualize the sample. The algorithm used does not have any influence on the sample chromaticity and the generated image can be obtained with full chromaticity.

Published in:

Review of Scientific Instruments  (Volume:79 ,  Issue: 11 )