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We control in vitro gene expression, using DNA (gene) sequences immobilized on miniaturized heaters. For this, electronically addressable indium–tin–oxide heating pads, 100 μm2 in size, are fabricated on a glass substrate to locally define temperatures and thus control transcription and translation of DNA gene sequences attached to the pad. Wheat germ cell extract is the reaction medium. We use luciferase gene and monitor its temperature-controlled expression by luminescence. Various constructs are tested yielding either luciferase free or bound to the coding DNA. Control of protein production at precise sites and rates, using temperature, opens the possibility to engineer various protein networks with different time scales. Working with constructs where the protein stays bound, gene product micro-arrays can be realized. © 2000 American Institute of Physics.