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Nonoptically probing near-field microscopy for the observation of biological living specimens

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8 Author(s)
Kawata, Y. ; Faculty of Engineering, Shizuoka University, Johoku, Hamamatsu 432-8581, Japan ; Murakami, Manabu ; Egami, Chikara ; Sugihara, O.
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We present the observation of living specimens with subwavelength resolution by using the nonoptically probing near-field microscopy we have developed recently. In the near-field microscope, the optical field distributions near the specimens are recorded as the surface topography of a photosensitive film, and the topographical distributions are readout with an atomic-force microscopy. Since the near-field microscope does not require the scanning of a probe tip for illumination or detection or scattering of light, it is possible to observe moving biological specimens and fast phenomena. We demonstrate the observation of a moving paramecium and euglena gracilis with subwavelength resolution. The observation of the nucleus inside a euglena cell was also demonstrated. © 2001 American Institute of Physics.

Published in:
Applied Physics Letters  (Volume:78 ,  Issue: 15 )

Date of Publication: Apr 2001

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