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Single DNA molecule grafting and manipulation using a combined atomic force microscope and an optical tweezer

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2 Author(s)
Shivashankar, G.V. ; Center for Studies in Physics and Biology, The Rockefeller University, New York, New York 10021 ; Libchaber, A.

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In this letter, we report on spatially selecting and grafting a DNA-tethered bead to an atomic force microscope (AFM) cantilever, using an optical tweezer. To quantify this technique, we measure force versus extension of a single DNA molecule using AFM. For such studies, we have developed a micromanipulation approach by combining an AFM, an optical tweezer, and visualization setup. The ability to select a single DNA polymer and specifically graft it to a localized position on a substrate opens up new possibilities in biosensors and bioelectronic devices. © 1997 American Institute of Physics.

Published in:

Applied Physics Letters  (Volume:71 ,  Issue: 25 )