Nonlinear filtering for the estimation of glycated hemoglobin in the presence of fetal hemoglobin using microcolumn ion-exchange chromatography

An improved method based on nonlinear least-squares to estimate the percentage concentration of glycated hemoglobin levels HbA1ab, HbA1c, HbF, and other Hb variants is presented. If the existing method is enhanced with the improved method, the use of microcolumn ion-exchange chromatography for estimating glycated hemoglobin levels even for patients with elevated fetal hemoglobin (HbF) would provide a more realistic estimate.