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Single-molecule signal enhancement using a high-impedance ground plane substrate

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5 Author(s)
Ray, Krishanu ; Department of Applied Physics, Yale University, New Haven, Connecticut 06520 ; Mason, Michael D. ; Yang, Celeste ; Li, Zhipan
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We introduce a “high-impedance ground plane” (HIGP) substrate for use in single-molecule imaging applications. The HIGP is a quarter-wave-thick layer of dielectric material is deposited over a regular metallic mirror. Comparison of confocal fluorescence images of single DiI molecules on HIGP substrates and on glass substrates indicates a dramatic increase in the number of collected photons for the HIGP substrate. Signal enhancement ranges from a factor of 8 at low excitation powers to a lifetime-limited factor of 2 at higher powers.

Published in:

Applied Physics Letters  (Volume:85 ,  Issue: 23 )

Date of Publication:

Dec 2004

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