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Phase-resolved optical Doppler tomography (ODT), an imaging technique based on low coherence interferometry, is presented as a tool to perform high-resolution cross-sectional imaging of fluidic flow in microchannels with high velocity sensitivity. To demonstrate ODT as a tool, electro-osmotic flow (EOF) was investigated, observing cross-sectional images of bidirectional flow within a microfluidic channel and pulsating flow when driven by a pulsed electrical field. ODT demonstrates great promise as a tool for studying the effects of microchannel surface modifications on biological sample flow and optimizing microfluidic device design.